Radioactive Human Lymphoblastoid Interferon
نویسندگان
چکیده
منابع مشابه
Human lymphoblastoid interferon. Large scale production and partial purification.
Human lymphoblastoid interferon was produced on an 800-liter scale (2.6 X 10(9) units) by induction of Namalva cells with Newcastle disease virus, strain B1. The interferon was partially purified by anti-leukocyte interferon affinity chromatography, sulfopropyl Sephadex ion exchange chromatography, isoelectric focusing, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Recovery of ...
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Optimum conditions for growth and interferon production by a human lymphoblastoid cell line, Namalva, have been studied. Adaptation to large-scale production is possible utilizing either Sendai virus or Newcastle disease virus. Priming of cultures before induction is unnecessary. The interferon produced has properties similar to human leukocyte interferon. The production of lymphoblastoid inter...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1982
ISSN: 0014-2956
DOI: 10.1111/j.1432-1033.1982.tb06714.x